Resident Evil 4
So you've just fought your way through dozens of angry villagers, your helicopter has been shot down, and now you've found yourself backed into a corner. Why not take refuge in this dark, spooky, not-ominous-at-all castle?
As many times as I've played RE4, nothing the game throws at you really phases me anymore. But man, oh man, that first time I went through the castle it had me on edge the entire visit. Whether it was not knowing where the Zealots were coming from (all the while chanting "Muere, muere, muere", "MATALO!!", and other Spanish phrases I don't understand), being trapped in a tiny cage with a guy whose Wolverine claws touch the floor, or trekking through a hedge maze (a freakin' hedge maze!) with zombie wolves constantly popping out at you, it was truly a terrifying experience.
All the while you are taunted by the castle's owner, Ramón Salazar, a diminutive young man with the appearance of a senior. He lures you into all kinds of traps and sends monsters after you, and just when you think you've reached him, part two of the castle starts. Then you reach the sewers, and you're forced to deal with his bodyguard (possibly the hardest fight in the game if you don't use/mess up the liquid nitrogen tanks) and track Salazar down once again.
The spookiest part of this area, however, is when the player takes control of Ashley, and you have no means of defending yourself aside from a couple of conveniently placed lanterns. The castle remains to this day my favorite area of the entire game. Someday when Media Tsunami really takes off and we become millionaires, I'll attempt to replicate for my own purposes. Mwahahaha!!!!